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Biomimetic triblock copolymer membrane arrays: a stable template for functional membrane proteins

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Authors:
  • Gonzalez-Perez, A. ;
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    unknown
  • Jensen, Karin Bagger Stibius ;
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    Department of Physics, Technical University of Denmark
  • Vissing, Thomas ;
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    Department of Chemistry, Technical University of Denmark
  • Helix Nielsen, Claus ;
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    Orcid logo0000-0002-9392-7133
    Biophysics and Fluids, Department of Physics, Technical University of Denmark
  • Mouritsen, Ole G.
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    Department of Chemistry, Technical University of Denmark
DOI:
10.1021/la902417m
Abstract:
It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.
Type:
Journal article
Language:
English
Published in:
Langmuir, 2009, Vol 25, Issue 18, p. 10447-10450
Keywords:
Main Research Area:
Science/technology
Publication Status:
Published
Review type:
Peer Review
Submission year:
2009
Scientific Level:
Scientific
ID:
5254818

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