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Genome Sequencing

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Authors:
  • Sato, Shusei ;
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    Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-Ku, Sendai, 980-8577, Japan
  • Andersen, Stig Uggerhøj
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    Orcid logo0000-0002-1096-1468
    Department of Molecular Biology and Genetics - Plant Molecular Biology, Department of Molecular Biology and Genetics, Science and Technology, Aarhus University
Editor:
Tabata, Satoshi, Stougaard, Jens
DOI:
10.1007/978-3-662-44270-8_4
Abstract:
The current Lotus japonicus reference genome sequence is based on a hybrid assembly of Sanger TAC/BAC, Sanger shotgun and Illumina shotgun sequencing data generated from the Miyakojima-MG20 accession. It covers nearly all expressed L. japonicus genes and has been annotated mainly based on transcriptional evidence. Analysis of repetitive sequences suggests that they are underrepresented in the reference assembly, reflecting an enrichment of gene-rich regions in the current assembly. Characterization of Lotus natural variation by resequencing of L. japonicus accessions and diploid Lotus species is currently ongoing, facilitated by the MG20 reference sequence
ISBN:
9783662442692
Type:
Book chapter
Language:
English
Published in:
Lotus Japonicus Genome, 2014, p. 35-40
Main Research Area:
Science/technology
Publication Status:
Published
Series:
Compendium of Plant Genomes
Review type:
Undetermined
Publisher:
Springer
Submission year:
2014
Scientific Level:
Scientific
ID:
274937555

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