• EN
  • DA

Danish NationalResearch Database

  • Publications
  • Researchers
Example Finds records
water{} containing the word "water".
water supplies"{}" containing the phrase "water supplies".
author:"Doe, John"author:"{}" containing the phrase "Doe, John" in the author field.
title:IEEEtitle:{} containing the word "IEEE" in the title field.
bech{} containing the word "bech".
marie bech"{}" containing the phrase "marie bech".
orcid:0000-0002-5429-5292orcid:{} Having a particular ORCID
Need more help? Advanced search tutorial
  • Selected (0)
  • History

Polymorphonuclear leukocytes restrict growth of <i>Pseudomonas aeruginosa</i> in the lungs of cystic fibrosis patients

    • Save to Mendeley
    • Export to BibTeX
    • Export to RIS
    • Email citation
Authors:
  • Kragh, Kasper Nørskov ;
    Close
    Orcid logo0000-0002-7662-9118
    Department of Immunology and Microbiology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Alhede, Morten ;
    Close
    Department of Immunology and Microbiology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Jensen, Peter Østrup ;
    Close
    Bacteriology, Institute for Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Moser, Claus Ernst ;
    Close
    Orcid logo0000-0002-7664-8570
    Department of Immunology and Microbiology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Scheike, Thomas ;
    Close
    Orcid logo0000-0002-2148-4740
    Section of Biostatistics, Department of Public Health, Faculty of Health and Medical Sciences, Københavns Universitet
  • Jacobsen, Carsten S. ;
    Close
    Geological Survey of Denmark and Greenland
  • Poulsen, Steen Seier ;
    Close
    Orcid logo0000-0003-4419-4911
    Endocrinology and Metabolism, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, Københavns Universitet
  • Eickhardt-Sørensen, Steffen Robert ;
    Close
    Department of Immunology and Microbiology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Trostrup, Hannah ;
    Close
    Rigshospitalet
  • Christoffersen, Lars ;
    Close
    Rigshospitalet
  • Hougen, Hans Petter ;
    Close
    Section of Forensic Pathology, Department of Forensic Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Rickelt, Lars F. ;
    Close
    Marine Biology, Department of Biology, Faculty of Science, Københavns Universitet
  • Kühl, Michael ;
    Close
    Orcid logo0000-0002-1792-4790
    Marine Biology, Department of Biology, Faculty of Science, Københavns Universitet
  • Høiby, Niels ;
    Close
    Orcid logo0000-0002-1347-725X
    Department of Immunology and Microbiology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Bjarnsholt, Thomas
    Close
    Orcid logo0000-0002-8003-7414
    Department of Immunology and Microbiology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
DOI:
10.1128/IAI.01969-14
Abstract:
Cystic fibrosis (CF) patients have increased susceptibility to chronic lung infections by Pseudomonas aeruginosa, but the ecophysiology within the CF lung during infections is poorly understood. The aim of this study was to elucidate the in vivo growth physiology of P. aeruginosa within lungs of chronically infected CF patients. A novel, quantitative peptide nucleic acid (PNA) fluorescence in situ hybridization (PNA-FISH)-based method was used to estimate the in vivo growth rates of P. aeruginosa directly in lung tissue samples from CF patients and the growth rates of P. aeruginosa in infected lungs in a mouse model. The growth rate of P. aeruginosa within CF lungs did not correlate with the dimensions of bacterial aggregates but showed an inverse correlation to the concentration of polymorphonuclear leukocytes (PMNs) surrounding the bacteria. A growth-limiting effect on P. aeruginosa by PMNs was also observed in vitro, where this limitation was alleviated in the presence of the alternative electron acceptor nitrate. The finding that P. aeruginosa growth patterns correlate with the number of surrounding PMNs points to a bacteriostatic effect by PMNs via their strong O2 consumption, which slows the growth of P. aeruginosa in infected CF lungs. In support of this, the growth of P. aeruginosa was significantly higher in the respiratory airways than in the conducting airways of mice. These results indicate a complex host-pathogen interaction in chronic P. aeruginosa infection of the CF lung whereby PMNs slow the growth of the bacteria and render them less susceptible to antibiotic treatment while enabling them to persist by anaerobic respiration.
Type:
Journal article
Language:
English
Published in:
Infection and Immunity, 2014, Vol 82, Issue 11, p. 4477-4486
Keywords:
Adult; Animals; Biofilms; Cystic Fibrosis; Female; Humans; In Situ Hybridization, Fluorescence; Lung Diseases; Male; Mice; Mice, Inbred BALB C; Neutrophils; Peptide Nucleic Acids; Pseudomonas Infections; Pseudomonas aeruginosa; Journal Article; Research Support, Non-U.S. Gov't
Main Research Area:
Medical science
Publication Status:
Published
Review type:
Peer Review
Submission year:
2014
Scientific Level:
Scientific
ID:
269923990

Full text access

  • Doi Get publisher edition via DOI resolver
Checking for on-site access...

On-site access

At institutions

  • Copenhagen university.en
  • Capital region

Metrics

Feedback

Sitemap

  • Search
    • Statistics
    • Tutorial
    • Data
    • FAQ
    • Contact
  • About
    • Institutions
    • Release History
    • Cookies and Personal Data
  • Open Access
    • The Danish Open Access Indicator

Copyright © 1998–2018.

Fivu en