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Pyrolyzed Photoresist Electrodes for Integration in Microfluidic Chips for Transmitter Detection from Biological Cells

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Authors:
  • Larsen, Simon Tylsgaard ;
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    Department of Micro- and Nanotechnology, Technical University of Denmark
  • Argyraki, Aikaterini ;
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    Orcid logo0000-0002-9865-164X
    Department of Micro- and Nanotechnology, Technical University of Denmark
  • Amato, Letizia ;
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    Department of Micro- and Nanotechnology, Technical University of Denmark
  • Tanzi, Simone ;
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    Department of Micro- and Nanotechnology, Technical University of Denmark
  • Keller, Stephan Sylvest ;
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    Orcid logo0000-0003-4108-1305
    Department of Micro- and Nanotechnology, Technical University of Denmark
  • Rozlosnik, Noemi ;
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    Department of Micro- and Nanotechnology, Technical University of Denmark
  • Taboryski, Rafael J.
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    Orcid logo0000-0003-2491-1098
    Department of Micro- and Nanotechnology, Technical University of Denmark
DOI:
10.1149/2.005305eel
Abstract:
In this study, we show how pyrolyzed photoresist carbon electrodes can be used for amperometric detection of potassium-induced transmitter release from large groups of neuronal PC 12 cells. This opens the way for the use of carbon film electrodes in microfabricated devices for neurochemical drug screening applications. We also investigated the effect of using two different photoresists for fabrication of pyrolyzed photoresist electrodes. We observed a significant difference in the cross-sectional profile of band electrodes made of AZ 4562 and AZ 5214 photoresist. This difference can be explained by the difference in photoresist viscosity. By adding a soft bake step to the fabrication procedure, the flatness of pyrolyzed AZ 5214 electrodes could be improved which would facilitate their integration in microfluidic chip devices.
Type:
Journal article
Language:
English
Published in:
E C S Electrochemistry Letters, 2013, Vol 2, Issue 5
Main Research Area:
Science/technology
Submission year:
2013
ID:
239160275

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