• EN
  • DA

Danish NationalResearch Database

  • Publications
  • Researchers
Example Finds records
water{} containing the word "water".
water supplies"{}" containing the phrase "water supplies".
author:"Doe, John"author:"{}" containing the phrase "Doe, John" in the author field.
title:IEEEtitle:{} containing the word "IEEE" in the title field.
bech{} containing the word "bech".
marie bech"{}" containing the phrase "marie bech".
orcid:0000-0002-5429-5292orcid:{} Having a particular ORCID
Need more help? Advanced search tutorial
  • Selected (0)
  • History

Whole blood samples for adrenocorticotrophic hormone measurement can be stored at room temperature for 4 hours

    • Save to Mendeley
    • Export to BibTeX
    • Export to RIS
    • Email citation
Authors:
  • Christensen, Mette ;
    Close
    Department of Business Development and Technology, Aarhus BSS, Aarhus University
  • Madsen, Rikke Fogt ;
    Close
    a Faculty of Health Science , VIA University College , Aarhus , Denmark.
  • Møller, Line Rosengreen ;
    Close
    a Faculty of Health Science , VIA University College , Aarhus , Denmark.
  • Knudsen, Cindy Soendersoe ;
    Close
    Department of Clinical Medicine - Clinical Biochemistry, Department of Clinical Medicine, Health, Aarhus University
  • Samson, Mie Hessellund
    Close
    Department of Clinical Medicine - Clinical Biochemistry, Department of Clinical Medicine, Health, Aarhus University
DOI:
10.1080/00365513.2016.1230887
Abstract:
INTRODUCTION: The aim of this study was to investigate and compare the stability of adrenocorticotrophic hormone (ACTH) in whole blood stored on ice and at room temperature for up to 48 hours. This study differs from previous studies by a larger data material. MATERIALS AND METHODS: EDTA-blood samples from 30 patients were collected, aliquoted and stored on ice or at room temperature for 0, 2, 4, 24, or 48 h before centrifugation, and the plasma was stored frozen until analysis. All samples were analyzed using an automated electrochemiluminescence immunoassay on cobas 6000 e601. The change in ACTH concentration was illustrated as ACTH recovery compared to standard conditions defined as samples stored immediately on ice, centrifuged and plasma frozen within 1 h. A change in ACTH concentration of more than 10% was considered to be of clinical relevance. RESULTS: The results showed no clinically relevant change in ACTH recovery for up to 4 h compared to standard conditions. For samples stored at room temperature for 4 h, a significant (p < .0001) relative mean change in ACTH concentrations of -4.3% was observed. CONCLUSION: The comparison between samples stored at room temperature for up to 4 h and standard conditions showed that ACTH samples do not require cooling until centrifugation, if a mean difference in ACTH concentration of -4.3%, between the individual results, can be accepted.
Type:
Journal article
Language:
English
Published in:
Scandinavian Journal of Clinical and Laboratory Investigation, 2016, Vol 76, Issue 8, p. 653-656
Keywords:
ACTH; corticotrophin; hormones; ice; plasma; pre-analytical; protein stability; storage; temperature
Main Research Area:
Medical science
Publication Status:
Published
Review type:
Peer Review
Submission year:
2016
Scientific Level:
Scientific
ID:
2346700075

Full text access

  • Doi Get publisher edition via DOI resolver
Checking for on-site access...

On-site access

At institution

  • Aarhus university.en

Metrics

Feedback

Sitemap

  • Search
    • Statistics
    • Tutorial
    • Data
    • FAQ
    • Contact
  • About
    • Institutions
    • Release History
    • Cookies and Personal Data
  • Open Access
    • The Danish Open Access Indicator

Copyright © 1998–2018.

Fivu en