Continuous Exposure Of <i>Vibrio Anguillarum</i> To Tropodithietic Acid: Genetic Changes And Influence On Virulence
- Authors:
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Rasmussen, Bastian Barker
;
Close
0000-0002-2523-9304
Department of Biotechnology and Biomedicine, Technical University of Denmark - D'Alvise, Paul ;
- Grotkjær, Torben ;
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Bentzon-Tilia, Mikkel
;
Close0000-0002-7888-9845
Bacterial Ecophysiology and Biotechnology, Department of Systems Biology, Technical University of Denmark -
Gram, Lone
Close0000-0002-1076-5723
Bacterial Ecophysiology and Biotechnology, Department of Systems Biology, Technical University of Denmark
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Rasmussen, Bastian Barker
;
- Abstract:
- Introduction: The fish pathogen Vibrio anguillarum is a major problem in aquaculture causing Vibriosis. Bacteria of the Roseobacter clade can antagonize pathogenic vibrios in cultures in live feed such as microalgae, rotifers and Artemia, as well as in fish larvae. Therefore, roseobacters could be promising as probiotics in fish rearing. Production of the antibacterial compound tropodithietic acid (TDA) by roseobacters is key in the antagonism of vibrios. However, the effects of continuous exposure to TDA on V. anguillarum remain unknown. The purpose of this study was to investigate how prolonged TDA exposure affects V. anguillarum focusing on the development of resistance towards TDA and changes in virulence.Methods: Seven lineages of V. anguillarum were exposed to increasing TDA concentrations over 300-400 generations and were subsequently genome sequenced. Virulence of the lineages is currently being tested in fish cell infection trials.Results: Following exposure, four linages reached 1.75 x wild-type MIC and three reached 1.5 x wild-type MIC. Genome sequencing revealed no major changes in the genomes of the lineages. The only virulence-related gene affected was fliM, encoding a flagella motor switch protein. However, mutations in this gene were observed in non-exposed controls as well.Conclusions: In conclusion, TDA resistance does not appear to develop, and the virulence genes of V. anguillarum are unaffected by TDA exposure, supporting the application of TDA-producing roseobacters as probiotics in aquaculture.
- Type:
- Conference abstract
- Language:
- English
- Published in:
- Danish Microbiological Society Annual Congress 2015: Programme & Abstracts, 2015, p. 72-72
- Main Research Area:
- Science/technology
- Publication Status:
- Published
- Review type:
- Peer Review
- Conference:
- The Danish Microbiological Society Annual Congress 2015
- Submission year:
- 2015
- Scientific Level:
- Scientific
- ID:
- 2289345005
