We introduce full three-dimensional tracking of particles in an acoustophoresis microchannel using Astigmatism Particle Tracking Velocimetry (APTV) . For the first time the interaction between acoustic streaming and the primary acoustic radiation force in microchannel acoustophoresis are examined in three dimensions. We have quantified the velocity of particles driven by the primary acoustic radiation force and acoustic streaming, respectively, using 0.5-μm and 5-μm particles. Increased ultrasound frequency and lowered viscosity of the medium reduced the influence of acoustic streaming relative to the influence from the acoustic radiation force. The current study opens the route to optimized acoustophoretic system design and operation to enable manipulation of small biological components such as spores, bacteria and viruses.
Proceedings of the Microtas 2012 Conference, 2012, p. 1480-1482
Main Research Area:
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)