The most widely used method to study individuals’ public service motivation is surveys. The validity and inferential power of such studies may, however, be harmed by survey participation bias, if highly public service motivated individuals are overrepresented among respondents. This paper examines the methodological critique of survey participation bias raised against the public service motivation literature and examines whether public service motivation increases the propensity to respond to surveys. By tracking more than 3,000 public service providers’ participation in a three-wave panel survey, our analysis shows that public service motivation does have a positive effect on survey participation. This suggests that future studies should pay attention to the characteristics of respondents/non-respondents and consider weighting the sample.