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Determination of beta-defensin genomic copy number in different populations

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Authors:
  • Fode, Peder ;
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    Institut for Klinisk Medicin
  • Jespersgaard, Cathrine ;
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    LUKKET: Wilhelm Johannsen Center, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
  • Hardwick, Robert J ;
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    unknown
  • Bogle, Helen ;
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    unknown
  • Theisen, Michael ;
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    Orcid logo0000-0003-4412-1392
    GLURP team, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Dodoo, Daniel ;
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    unknown
  • Lenicek, Martin ;
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    unknown
  • Vitek, Libor ;
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    unknown
  • Vieira, Ana ;
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    unknown
  • Freitas, Joao ;
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    unknown
  • Andersen, Paal Skytt ;
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    unknown
  • Hollox, Edward J
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    unknown
Subtitle:
a comparison of three methods
DOI:
10.1371/journal.pone.0016768
Abstract:
There have been conflicting reports in the literature on association of gene copy number with disease, including CCL3L1 and HIV susceptibility, and ß-defensins and Crohn's disease. Quantification of precise gene copy numbers is important in order to define any association of gene copy number with disease. At present, real-time quantitative PCR (QPCR) is the most commonly used method to determine gene copy number, however the Paralogue Ratio Test (PRT) is being used in more and more laboratories.
Type:
Journal article
Language:
English
Published in:
P L O S One, 2011, Vol 6, Issue 2
Keywords:
Base Sequence; Chromosome Mapping; Cohort Studies; Czech Republic; Denmark; Gene Dosage; Genetic Predisposition to Disease; Genetics, Population; Genome, Human; Ghana; Great Britain; Humans; Molecular Sequence Data; Population; Portugal; beta-Defensins
Main Research Area:
Medical science
Publication Status:
Published
Review type:
Peer Review
Submission year:
2011
Scientific Level:
Scientific
ID:
180284147

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