Determination of beta-defensin genomic copy number in different populations: a comparison of three methods

Peder Fode; Cathrine Jespersgaard; Robert J Hardwick; Helen Bogle; Michael Theisen; Daniel Dodoo; Martin Lenicek; Libor Vitek; Ana Vieira; Joao Freitas; Paal Skytt Andersen; Edward J Hollox

doi:10.1371/journal.pone.0016768

Determination of beta-defensin genomic copy number in different populations

Authors:

Fode, Peder ;
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Institut for Klinisk Medicin
Jespersgaard, Cathrine ;
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LUKKET: Wilhelm Johannsen Center, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
Hardwick, Robert J ;
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unknown
Bogle, Helen ;
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unknown
Theisen, Michael ;
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GLURP team, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Københavns Universitet
Dodoo, Daniel ;
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unknown
Lenicek, Martin ;
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Vitek, Libor ;
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unknown
Vieira, Ana ;
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Freitas, Joao ;
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unknown
Andersen, Paal Skytt ;
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Hollox, Edward J
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unknown

Subtitle:

a comparison of three methods

DOI:

10.1371/journal.pone.0016768

Abstract:

There have been conflicting reports in the literature on association of gene copy number with disease, including CCL3L1 and HIV susceptibility, and ß-defensins and Crohn's disease. Quantification of precise gene copy numbers is important in order to define any association of gene copy number with disease. At present, real-time quantitative PCR (QPCR) is the most commonly used method to determine gene copy number, however the Paralogue Ratio Test (PRT) is being used in more and more laboratories.

Type:

Journal article

Language:

English

Published in:

P L O S One, 2011, Vol 6, Issue 2

Keywords:

Base Sequence; Chromosome Mapping; Cohort Studies; Czech Republic; Denmark; Gene Dosage; Genetic Predisposition to Disease; Genetics, Population; Genome, Human; Ghana; Great Britain; Humans; Molecular Sequence Data; Population; Portugal; beta-Defensins

Main Research Area:

Medical science

Submission year:

2011

ID:

180284147

Determination of beta-defensin genomic copy number in different populations

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