Biophysical properties of phenyl succinic acid derivatised hyaluronic acid

Maria Teresa Neves-Petersen; Søren Klitgaard; Esben Skovsen; Steffen B Petersen; Kristoffer Tømmeraas; Khadija Schwach-Abdellaoui

doi:10.1007/s10895-009-0570-z

Biophysical properties of phenyl succinic acid derivatised hyaluronic acid

Authors:

Neves-Petersen, Maria Teresa ;
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Department of Physics and Nanotechnology, The Faculty of Engineering and Science, Aalborg University
Klitgaard, Søren ;
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Department of Physics and Nanotechnology, The Faculty of Engineering and Science, Aalborg University
Skovsen, Esben ;
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0000-0001-9990-367X
The Faculty of Engineering and Science, Aalborg University
Petersen, Steffen B ;
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The Faculty of Medicine, Aalborg University
Tømmeraas, Kristoffer ;
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unknown
Schwach-Abdellaoui, Khadija
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unknown

DOI:

10.1007/s10895-009-0570-z

Abstract:

Modification of hyaluronic acid (HA) with aryl succinic anhydrides results in new biomedical properties of HA as compared to non-modified HA, such as more efficient skin penetration, stronger binding to the skin, and the ability to blend with hydrophobic materials. In the present study, hyaluronic acid has been derivatised with the anhydride form of phenyl succinic acid (PheSA). The fluorescence of PheSA was efficiently quenched by the HA matrix. HA also acted as a singlet oxygen scavenger. Fluorescence lifetime(s) of PheSA in solution and when attached to the HA matrix has been monitored with ps resolved streak camera technology. Structural and fluorescence properties changes induced on HA-PheSA due to the presence of singlet oxygen were monitored using static light scattering (SLS), steady state fluorescence and ps time resolved fluorescence studies. SLS studies provided insight into the depolymerisation kinetics of PheSA derivatised HA matrix in the presence of singlet oxygen. Time resolved fluorescence studies grave insight into the dynamics of the reaction mechanisms induced on HA-PheSA by singlet oxygen. These studies provided insight into the medical relevance of PheSA derivatised HA: its capacity of scavenging singlet oxygen and of quenching PheSA fluorescence. These studies revealed that HA-PheSA is a strong quencher of electronic excited state PheSA and acts as a scavenger of singlet oxygen, thus medical applications of this derivatised form of HA may protect tissues and organs, such as skin, against reactive oxygen species damage.

Type:

Journal article

Language:

English

Published in:

Journal of Fluorescence, 2010, Vol 20, Issue 2, p. 483-92

Keywords:

Anhydrides; Fluorescence; Hyaluronic Acid; Kinetics; Light; Scattering, Radiation; Singlet Oxygen; Succinic Acids; Time Factors

Main Research Area:

Science/technology

Publication Status:

Published

Review type:

Peer Review

Submission year:

2010

Scientific Level:

Scientific

ID:

155918

Biophysical properties of phenyl succinic acid derivatised hyaluronic acid

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