{"controller"=>"catalog", "action"=>"show", "locale"=>"da", "id"=>"260932500"}
  • EN
  • DA

Den DanskeForskningsdatabase

  • Søgning Publikationer & Forskere
  • Open Access Indikator
  • Publikationer
  • Forskere
Eksempel Finder dokumenter
water{} som indeholder ordet "water".
water supplies"{}" som indeholder tekststrengen "water supplies".
forfatter:"Doe, John"forfatter:"{}" som indeholder tekststrengen "Doe, John" i feltet: Forfatter.
titel:IEEEtitel:{} som indeholder ordet "IEEE" i feltet: Titel.
Brug for mere hjælp? Avanceret søgning
  • Udvalgte (0)
  • Historik

Recovery of cellulase activity after ethanol stripping in a novel pilot-scale unit

    • Gem til Mendeley
    • Eksportér til BibTeX
    • Eksportér til RIS
    • Email citering
Forfattere:
  • Skovgaard, Pernille Anastasia ;
    Close
    University of Copenhagen
  • Christensen, Børge Holm ;
    Close
    Holm Christensen Biosystemer Aps
  • Felby, Claus ;
    Close
    University of Copenhagen
  • Jørgensen, Henning
    Close
    Orcid logo0000-0003-1220-6893
    Department of Chemical and Biochemical Engineering, Technical University of Denmark
DOI:
10.1007/s10295-014-1413-8
Resumé:
Recycling of enzymes has a potential interest during cellulosic bioethanol production as purchasing enzymes is one of the largest expenses in the process. By recycling enzymes after distillation, loss of sugars and ethanol are avoided, but depending on the distillation temperature, there is a potential risk of enzyme degradation. Studies of the rate of enzyme denaturation based on estimation of the denaturation constant K D was performed using a novel distillation setup allowing stripping of ethanol at 50–65 °C. Experiments were performed in a pilot-scale stripper, where the effect of temperature (55–65 °C) and exposure to gas–liquid and liquid–heat transmission interfaces were tested on a mesophilic and thermostable enzyme mixture in fiber beer and buffer. Lab-scale tests were included in addition to the pilot-scale experiments to study the effect of shear, ethanol concentration, and PEG on enzyme stability. When increasing the temperature (up to 65 °C) or ethanol content (up to 7.5 % w/v), the denaturation rate of the enzymes increased. Enzyme denaturation occurred slower when the experiments were performed in fiber beer compared to buffer only, which could be due to PEG or other stabilizing substances in fiber beer. However, at extreme conditions with high temperature (65 °C) and ethanol content (7.5 % w/v), PEG had no enzyme stabilizing effect. The novel distillation setup proved to be useful for maintaining enzyme activity during ethanol extraction.
Type:
Tidsskrift-artikel
Sprog:
Engelsk
Udgivet i:
Journal of Industrial Microbiology and Biotechnology, 2014, Vol 41, Issue 4, p. 637-646
Emneord:
Distillation; Enzyme recycling; Ethanol; Gas-liquid interfaces; Thermostable cellulases; Gas–liquid interfaces; Denaturation constant (KD)
Hovedforskningsområde:
Science/technology
Publikationsstatus:
Publiceret
Review type:
Peer Review
Indberetningsår:
2014
Videnskabeligt niveau:
Videnskabelig
ID:
260932500

Fuldtekstadgang

  • Openaccess Online andetsteds
  • Doi Hent forlagsudgave gennem DOI-opslag
Tjekker on-site adgang...

On-site adgang

Hos forskningsinstitutioner

  • Copenhagen university.da
  • Technical university of dk

Metrics

Feedback

Sitemap

  • Søgning
    • Statistik
    • Vejledning
    • Data
    • FAQ
    • Kontakt
  • Open Access
    • Oversigt
    • Udvikling
    • FAQ
    • Kontakt
  • Om Basen
    • Institutioner
    • Release historie
    • Cookie- og privatlivspolitik

Copyright © 1998–2017.

Fivu da