Bagger Jørgensen, Rikke4; Jensen, C. J.5; Andersen, B.5; Bothmer, R. von7
1 Ecosystems, Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark2 Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark3 Risø National Laboratory for Sustainable Energy, Technical University of Denmark4 Department of Environmental Engineering, Technical University of Denmark5 Technical University of Denmark6 Lund University7 Lund University
Callus was induced from hybrids between cultivated barley (Hordeum vulgare L. ssp. vulgare) and ten species of wild barley (Hordeum L.) as well as from one backcross line ((H. lechleri .times. H. vulgare) .times. H. vulgare). Successful callus induction and regeneration of plants were achieved from explants of young spikes on the barley medium J 25-8. The capacity for plant regeneration was dependent on the wild parental species. In particular, combinations with four related wild species, viz. H. jubatum, H. roshevitzii, H. lechleri, and H. procerum, regenerated high numbers of plants from calli.
Plant Cell, Tissue and Organ Culture, 1986, Vol 6, Issue 3, p. 199-208