1 Direktionen, Rigshospitalet, The Capital Region of Denmark2 Department of Endocrinology, Medical Center, Amager and Hvidovre Hospital, The Capital Region of Denmark3 Hæmatologisk Klinik, Finsencentret, Rigshospitalet, The Capital Region of Denmark4 unknown5 Medicinsk Afdeling
Polymorphonuclear leucocyte (PMN) ingestion of particles coated with lipopolysaccharide (LPS) from Escherichia coli was compared to other PMN functions in seven patients with insulin dependent diabetes mellitus (IDDM) during short-term controlled metabolic changes from normo- to hyperglycemia without ketoacidosis. Factors known to interfere with PMN functions were excluded. PMN ingestion of particles coated with both LPS and bovine serum albumin became reduced from normo- to hyperglycemia. PMN motility was impaired in IDDM, but did not seem to be affected by short-term changes in metabolic control. PMN metabolism did not change from normo-to hyperglycemia. Particle-uptake by diabetic PMN is impaired after short term hyperglycemia in the range normally occurring in diabetics in every-day life.