Nielsen, Jeppe Lund2; Schramm, Andreas5; Bernhard, Anne E.2; van den Engh, Gerrit J.4; Stahl, David A.2
1 Department of Biological Sciences, Microbiology, Faculty of Science, Aarhus University, Aarhus University2 Department of Civil and Environmental Engineering, University of Washington, Seattle, WA3 Department of Bioscience - Microbiology, Department of Bioscience, Science and Technology, Aarhus University4 Institute for Systems Biology, Seattle, WA5 Department of Bioscience - Microbiology, Department of Bioscience, Science and Technology, Aarhus University
FLOW CYTOMETRY-ASSISTED CLONING OF SPECIFIC SEQUENCE MOTIFS FROM COMPLEX 16S RRNA GENE LIBRARIES Jeppe L. Nielsen,1 Andreas Schramm,1,2 Anne E. Bernhard,1 Gerrit J. van den Engh,3 and David A. Stahl1* Department of Civil and Environmental Engineering, University of Washington,1 and Institute for Systems Biology,3 Seattle, Washington, and Department of Ecological Microbiology, University of Bayreuth, Bayreuth, Germany2 A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.
Appl. Environ. Microbiol, 2004, Vol 70, Issue 12, p. 7550-7554