Strains carrying deletions in the atp genes, encoding the H+-ATPase, were unable to grow on nonfermentable substrates such as succinate, whereas with glucose as the substrate the growth rate of an atp deletion mutant was surprisingly high (some 75-80% of wild-type growth rate). The rate of glucose and oxygen consumption of these mutants was increased compared to the wild-type rates. In order to analyze the importance of the H+-ATPase at its physiological level, the cellular concentration of H+-ATPase was modulated around the wild-type level, using genetically manipulated strains. The control coefficient by the H+-ATPase with respect to growth rate and catabolic fluxes was measured. Control on growth rate was absent at the wildtype concentration of H+-ATPase, independent of whether the substrate for growth was glucose or succinate. Control by the H+-ATPase on the catabolic fluxes, including respiration, was negative at the wild-type H+-ATPase level. Moreover, the turnover number of the individual H+-ATPase enzymes increased as the H+-ATPase concentration was lowered. The negative control by the H+-ATPase on catabolism may thus be involved in a homeostatic control of ATP synthesis and, to some extent, explain the zero control by the H+-ATPase on E. coli growth rate.
Journal of Bioenergetics and Biomembranes, 1995, Vol 27, Issue 6, p. 543-554
Escherichia coli; Gene Deletion; Genes, Bacterial; Glucose; Kinetics; Operon; Oxygen Consumption; Proton-Translocating ATPases; Succinates; EC 220.127.116.11 Proton-Translocating ATPases; IY9XDZ35W2 Glucose; BIOPHYSICS; CELL; PROTON-TRANSLOCATING ATPASE; NUCLEOTIDE-SEQUENCE; OXIDATIVE-PHOSPHORYLATION; ADENOSINE-TRIPHOSPHATASE; SYNTHASE; OPERON; GENES; PROMOTERS; SUBUNIT; MUTATIONS; oxidative phosphorylation; respiration; ATP; fluxes; Metabolic Control Analysis; Oxidative phosphorylation; ATP GENE; BACTERIAL GROWTH RATE; CATABOLIC FLUX; GENETICS; MUTATION; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; RESPIRATION; Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae  Escherichia coli; ATP 56-65-5Q, 42530-29-0Q, 94587-45-8Q, 111839-44-2Q; ATPASE 9000-83-3; OXYGEN 7782-44-7; 02506, Cytology - Animal; 10012, Biochemistry - Gases; 10060, Biochemistry studies - General; 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines; 10510, Biophysics - Bioenergetics: electron transport and oxidative phosphorylation; 10808, Enzymes - Physiological studies; 13003, Metabolism - Energy and respiratory metabolism; 16004, Respiratory system - Physiology and biochemistry; 31000, Physiology and biochemistry of bacteria; 31500, Genetics of bacteria and viruses; 36002, Medical and clinical microbiology - Bacteriology; Biochemistry and Molecular Biophysics; Bioenergetics; Cell Biology; Enzymology; Genetics; Infection; Metabolism; Physiology