1 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU2 Faculty of Science, SDU3 Department of Marketing & Management, Faculty of Business and Social Sciences, SDU4 unknown5 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU6 Department of Marketing & Management, Faculty of Business and Social Sciences, SDU
The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)2C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MS(n) analysis performed on a hybrid linear ion trap-orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry lends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants.
Journal of Mass Spectrometry, 2006, Vol 41, Issue 3, p. 372-89
Glycerophospholipids; Glycosphingolipids; Mass Spectrometry; Saccharomyces cerevisiae; Sphingolipids; Sphingosine