Dueholm, Morten Simonsen1; G. Marquesa, Irina6; Karst, Søren Michael2; D'Imperiob, Seth7; Tale, V. P.7; Lewis, Derrick7; Nielsen, Per Halkjær1; Nielsen, Jeppe Lund2
1 Department of Chemistry and Bioscience, The Faculty of Engineering and Science, Aalborg University, VBN2 Section of Biotechnology, The Faculty of Engineering and Science, Aalborg University, VBN3 The Faculty of Engineering and Science, Aalborg University, VBN4 Microbial Communities, The Faculty of Engineering and Science, Aalborg University, VBN5 EcoDesign, The Faculty of Engineering and Science, Aalborg University, VBN6 Center for Microbial Communities, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.7 Novozymes Biologicals, Salem, Virginia, USA.
Successful application of bioaugmentation for enhanced degradation of environmental pollutants is often limited by the lack of methods to monitor the survival and activity of individual bioaugmentation strains. However, recent advancements in sequencing technologies and molecular techniques now allow us to address these limitations. Here a complementing set of general applicable molecular methods are presented that provides detailed information on the performance of individual bioaugmentation strains under in situ conditions. The approach involves genome sequencing to establish highly specific qPCR and RT-qPCR tools for cell enumerations and expression of involved genes, stable isotope probing to follow growth on the target compounds and GFP-tagging to visualize the bioaugmentation strains directly in samples, all in combination with removal studies of the target compounds. The concept of the approach is demonstrated through a case study involving degradation of aromatic hydrocarbons in activated sludge augmented with the bioaugmentation strain Pseudomonas monteilii SB3078.
Bioresource Technology, 2015, Vol 186, p. 192-199
Bioaugmentation; In situ activity; BTEX; Survival; Pseudomonas monteilii