1 Department of Animal Science - Molecular nutrition and reproduction, Department of Animal Science, Science and Technology, Aarhus University2 Enzyme Development, DuPont Nutrition Biosciences Aps3 Dupont Industrial Biosciences4 Department of Animal Science - Molecular nutrition and reproduction, Department of Animal Science, Science and Technology, Aarhus University
The use of distiller dried grains with solubles (DDGS) as alternative to conventional animal feed for non-ruminants is challenged by the high content of non-starch polysaccharides and varying protein quality. In this study the enzymatic degradation of corn- and wheat DDGS was evaluated, in vitro, by use of four xylanases from two different glycoside hydrolase families, GH10 and GH11, along with protease and phytase. Wheat DDGS showed the highest degree of enzymatic degradation due to a lower degree of cell wall complexity compared with that of corn DDGS. For corn DDGS, the combination of xylanase and protease yielded the highest degree of enzymatic degradation, indicating close association of arabinoxylan and protein within the cell wall matrix. Collectively, the GH10 xylanase degraded DDGS more efficiently than the GH11 xylanases, due to the complexity of the substrate and the substrate affinity of this xylanase. The current in vitro results indicate a high potential of xylanase in combination with protease to efficiently degrade DDGS and promote nutrient release in diets for non-ruminant animals.
Biochemical Engineering Journal, 2015, Vol 98, p. 99-106