1 Neurobiologisk Forskning, Department of Molecular Medicine, Det Sundhedsvidenskabelige Fakultet, SDU2 Uppsala University3 Department of immunology, genetics, pathology and Neuroonkologi, Uppsala University, Uppsala, Sweden4 Department of Evolution and Development, Evolutionary Biology Centre, Uppsala University, Uppsala,Sweden.5 Department of Molecular Biology and genetics, Bosphorus university Istanbul, Turkey6 University of Milan7 Neurobiologisk Forskning, Department of Molecular Medicine, Det Sundhedsvidenskabelige Fakultet, SDU
The γ-aminobutyric acid (GABA) type B receptor has been implicated in glial cell development in the peripheral nervous system (PNS), although the exact function of GABA signaling is not known. To investigate GABA and its B receptor in PNS development and degeneration, we studied the expression of the GABAB receptor, GABA, and glutamic acid decarboxylase GAD65/67 in both development and injury in fetal dissociated dorsal root ganglia (DRG) cell cultures and in the rat sciatic nerve. We found that GABA, GAD65/67, and the GABAB receptor were expressed in premyelinating and nonmyelinating Schwann cells throughout development and after injury. A small population of myelinated sensory fibers displayed all of these molecules at the node of Ranvier, indicating a role in axon-glia communication. Functional studies using GABAB receptor agonists and antagonists were performed in fetal DRG primary cultures to study the function of this receptor during development. The results show that GABA, via its B receptor, is involved in the myelination process but not in Schwann cell proliferation. The data from adult nerves suggest additional roles in axon-glia communication after injury.
Journal of Neuroscience Research, 2015, Vol 3, Issue 93, p. 285-295
GABAB receptor; Schwann cells; GABA; Nodes of Ranvier; Peripheral Nervous System