Lysøe, Erik5; Harris, Linda J.6; Walkowiak, Sean6; Subramaniam, Rajagopal6; Divon, Hege H.7; Riiser, Even S.5; Llorens, Carlos8; Gabaldón, Toni12; Kistler, H. Corby10; Jonkers, Wilfried10; Kolseth, Anna-Karin11; Nielsen, Kristian Fog1; Thrane, Ulf1; Frandsen, Rasmus John Normand1
1 Department of Systems Biology, Technical University of Denmark2 Metabolomics Platform, Department of Systems Biology, Technical University of Denmark3 Fungal Physiology and Biotechnology, Department of Systems Biology, Technical University of Denmark4 Eucaryotic Molecular Cell Biology, Department of Systems Biology, Technical University of Denmark5 Norwegian Institute for Agricultural and Environmental Research6 Agriculture and Agri-Food Canada7 National Veterinary Institute8 Biotechvana9 Universitat Pompeu Fabra10 United States Department of Agriculture11 unknown12 Universitat Pompeu Fabra
Fusarium avenaceum is a fungus commonly isolated from soil and associated with a wide range of host plants. We present here three genome sequences of F. avenaceum, one isolated from barley in Finland and two from spring and winter wheat in Canada. The sizes of the three genomes range from 41.6-43.1 MB, with 13217-13445 predicted protein-coding genes. Whole-genome analysis showed that the three genomes are highly syntenic, and share>95% gene orthologs. Comparative analysis to other sequenced Fusaria shows that F. avenaceum has a very large potential for producing secondary metabolites, with between 75 and 80 key enzymes belonging to the polyketide, non-ribosomal peptide, terpene, alkaloid and indole-diterpene synthase classes. In addition to known metabolites from F. avenaceum, fuscofusarin and JM-47 were detected for the first time in this species. Many protein families are expanded in F. avenaceum, such as transcription factors, and proteins involved in redox reactions and signal transduction, suggesting evolutionary adaptation to a diverse and cosmopolitan ecology. We found that 20% of all predicted proteins were considered to be secreted, supporting a life in the extracellular space during interaction with plant hosts.