1 Department of Chemistry and Bioscience, The Faculty of Engineering and Science, Aalborg University, VBN2 Section of Biotechnology, The Faculty of Engineering and Science, Aalborg University, VBN3 The Faculty of Engineering and Science, Aalborg University, VBN4 Microbial Communities, The Faculty of Engineering and Science, Aalborg University, VBN5 unknown6 Ingeniørhøjskolen Aarhus Universitet - Bioprocesteknologi7 Institut for International Sundhed, Immunologi og Mikrobiologi
BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center. METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis. RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes. CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.
Journal of Cystic Fibrosis, 2014, Vol 13, Issue 6, p. 645-652