1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark3 Center for Nanomedicine and Theranostics, Center, Technical University of Denmark4 Novo Nordisk A/S
Interaction with lipid membranes and in vitro intestinal permeability
These results show that membrane interactions play a prominent role during intestinal translocation of an acylated peptide. Acylation benefits permeation for shorter and medium chains due to increased membrane interactions, however, for longer chains insertion in the membrane becomes dominant and hinders translocation, i.e. the peptides get 'stuck' in the cell membrane. Applying a transcellular absorption enhancer increases the dynamics of membrane insertion and detachment by fluidizing the membrane, thus facilitating its effects primarily on membrane associated peptides.