{"controller"=>"catalog", "action"=>"show", "id"=>"270693890"}
  • EN
  • DA

Danish NationalResearch Database

  • Search Publications & Researchers
  • Open Access Indicator
  • Publications
  • Researchers
Example Finds records
water{} containing the word "water".
water supplies"{}" containing the phrase "water supplies".
author:"Doe, John"author:"{}" containing the prase "Doe, John" in the author field.
title:IEEEtitle:{} containing the word "IEEE" in the title field.
Need more help? Advanced search tutorial
  • Selected (0)
  • History

Development of a One-Step Probe Based Molecular Assay for Rapid Immunodiagnosis of Infection with M. tuberculosis Using Dried Blood Spots

    • Save to Mendeley
    • Export to BibTeX
    • Export to RIS
    • Email citation
Authors:
  • Blauenfeldt, Thomas ;
    Close
    Clinical Research Centre, Amager and Hvidovre Hospital, The Capital Region of Denmark
  • Heyckendorf, Jan ;
    Close
    Clinical Infectious Diseases, German Center for Infection Research (DZIF) Tuberculosis Unit, Research Center Borstel, Borstel, Germany.
  • Graff Jensen, Sidse ;
    Close
    Thoracic Medicine and Infectious Disease, Department of, Nordsjællands Hospital, The Capital Region of Denmark
  • Lange, Christoph ;
    Close
    Clinical Infectious Diseases, German Center for Infection Research (DZIF) Tuberculosis Unit, Research Center Borstel, Borstel, Germany; Department of Medicine, University of Namibia School of Medicine, Windhoek, Namibia.
  • Drabe, Camilla ;
    Close
    Thoracic Medicine and Infectious Disease, Department of, Nordsjællands Hospital, The Capital Region of Denmark
  • Hermansen, Thomas S ;
    Close
    Thoracic Medicine and Infectious Disease, Department of, Nordsjællands Hospital, The Capital Region of Denmark
  • Thurah, Lena de ;
    Close
    Clinical Research Centre, Amager and Hvidovre Hospital, The Capital Region of Denmark
  • Lillebaek, Troels ;
    Close
    Department of Radiology, Centre for Functional and Diagnostic Imaging and research, Amager and Hvidovre Hospital, The Capital Region of Denmark
  • Eugen-Olsen, Jesper ;
    Close
    Orcid logo0000-0002-4630-4275
    Clinical Research Centre, Amager and Hvidovre Hospital, The Capital Region of Denmark
  • Seersholm, Niels ;
    Close
    Pulmonary Medicine, Herlev and Gentofte Hospital, The Capital Region of Denmark
  • Hoff, Søren ;
    Close
    Statens Serum Institut
  • Bonde, Jesper ;
    Close
    Pathology, Department of, Amager and Hvidovre Hospital, The Capital Region of Denmark
  • Ruhwald, Morten
    Close
    Clinical Research Centre, Amager and Hvidovre Hospital, The Capital Region of Denmark
DOI:
10.1371/journal.pone.0105628
Abstract:
BACKGROUND: Antigen specific release of IP-10 is the most promising alternative marker to IFN-γ for infection with M. tuberculosis. Compared to Interferon-γ release assays (IGRA), IP-10 is released in high levels enabling novel approaches such as field friendly dried blood spots (DBS) and molecular detection. AIM: To develop a robust IP-10 based molecular assay for the diagnosis of infection with M. tubercuolsis from whole blood and DBS. METHOD: We developed a one-step probe based multiplex RT-qPCR assay for detecting IP-10 and IFN-γ mRNA expression from whole blood and DBS samples. The assay was validated and applied for the diagnosis of M. tuberculosis infection in DBS samples from 43 patients with confirmed TB, 13 patients with latent TB and 96 presumed uninfected controls. In parallel, IP-10 and INF-γ levels were measured in Quantiferon (QFT-TB) plasma supernatants. RESULTS: IP-10 mRNA upregulation was detectable at 4 hours after stimulation (6 fold upregulation) peaking at 8 hours (108 fold upregulation). IFN-γ expression occurred in concert but levels were lower (peak 6.7 fold upregulation). IP-10 gene expression level was significantly higher in patients with tuberculosis (median 31.2, IQR 10.7-67.0) and persons with latent tuberculosis infection (LTBI) (41.2, IQR 9.8-64.9) compared to healthy controls (1.6, IQR 1.1-2.4; p<0.0001). The IP-10 mRNA and protein based tests had comparable diagnostic accuracy to QFT-TB, sensitivity (85% and 88% vs 85%) and specificity (96% and 96% vs 97%, p = ns.). CONCLUSION: We developed a rapid, robust and accurate molecular immunodiagnostic test for M. tuberculosis infection. By combining DBS based sample acquisition, mail or currier based sample transport with centralized molecular detection, this immunodiagnostic test concept can reduce the local technological requirements everywhere and make it possible to offer highly accurate immunodiagnostic tests in low resource settings.
Type:
Journal article
Language:
English
Published in:
P L O S One, 2014, Vol 9, Issue 9
Keywords:
Journal Article
Main Research Area:
Medical science
Publication Status:
Published
Review type:
Peer Review
Submission year:
2014
Scientific Level:
Scientific
ID:
270693890

Full text access

  • Doi Get publisher edition via DOI resolver
Checking for on-site access...

On-site access

At institution

  • Capital region

Metrics

Feedback

Sitemap

  • Search
    • Statistics
    • Tutorial
    • Data
    • FAQ
    • Contact
  • Open Access
    • Overview
    • Development
    • FAQ
    • Contact
  • About
    • Institutions
    • Release History
    • Cookies and privacy policy

Copyright © 1998–2018.

Fivu en