1 Food Safety and Zoonoses, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, Københavns Universitet2 Section of Microbiology, Department of Veterinary Disease Biology, Faculty of Life Sciences, Københavns Universitet3 Swiss Tropical and Public Health Institute4 Kasetsart University5 National Center for Parasitology, Entomology and Malaria Control6 University of Melbourne7 Section of Microbiology, Department of Veterinary Disease Biology, Faculty of Life Sciences, Københavns Universitet8 Food Safety and Zoonoses, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, Københavns Universitet9 University of Melbourne
BACKGROUND: A number of epidemiological studies have demonstrated Giardia as prevalent in both humans and dogs worldwide and have postulated the occurrence of anthroponotic, zoonotic and animal-specific cycles of transmission, which may be geographically and regionally unique in its epidemiology. The aim of this study was to utilise molecular tools to determine the prevalence and compare genotypes of Giardia duodenalis infecting humans and dogs living in a previously identified Giardia-endemic village in rural Cambodia in order to ascertain zoonotic transmission risk. FINDINGS: The prevalence of G. duodenalis in humans and dogs was 18.3% (40/218) and 10.6% (10/94) by PCR, respectively. Molecular characterisation of the small subunit of ribosomal RNA (SSU rRNA) gene, triose phosphate isomerase (TPI) gene and sub-assemblage characterisation of the glutamate dehydrogenase (gdh) gene placed 27.5% (11/40) of Giardia positive humans into assemblage AII and 72.5% (29/40) into assemblage BIII of G. duodenalis. In dogs, 20.0% (2/10) of Giardia-positive samples were characterised as G. duodenalis assemblage BIII, 40.0% (4/10) as assemblage C and 40.0% (4/10) as mix infection between assemblage C and D. CONCLUSION: Overall, just over 2% of dogs harboured potentially zoonotic assemblages of G. duodenalis in the studied communities and hence pose a minimal zoonotic risk for the transmission of Giardia to humans.
Parasites and Vectors, 2014, Vol 7
The Faculty of Health Science; Giardia duodenalis; Humans; Dogs; Zoonosis; Cambodia