Hogendorf, Wouter Frederik Johan4; Gisch, Nicolas3; Schwudke, Dominik3; Heine, Holger3; Bols, Mikael5; Pedersen, Christian Marcus5
1 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet2 Department of Chemistry, Faculty of Science, Københavns Universitet3 Leibniz-Center for Medicine and Biosciences4 Department of Chemistry, Faculty of Science, Københavns Universitet5 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet
The emergence of hypervirulent resistant strains have made Clostridium difficile a notorious nosocomial pathogen and has resulted in a renewed interest in preventive strategies, such as vaccines based on (synthetic) cell wall antigens. Recently, the structure of the lipoteichoic acid (LTA) of this species has been elucidated. Additionally, this LTA was found to induce the formation of protective antibodies against C. difficile in rabbits and mice. The LTA from C. difficile is isolated as a microheterogenous mixture, differing in size and composition, impeding any structure-activity relationship studies. To ensure reliable biological results, pure and well-defined synthetic samples are required. In this work the total synthesis of LTAs from C. difficile with defined chain length is described and the initial biological results are presented.
Chemistry: a European Journal, 2014, Vol 20, Issue 42, p. 13511-13516