1 Section for Transport Biology, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet2 Section for Plant Biochemistry, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet3 Section for Transport Biology, Department of Plant and Environmental Sciences, Faculty of Science, Københavns Universitet
Soluble nanoscale lipid bilayers, termed nanodiscs, are widely used in science for studying the membrane-anchored and integral membrane protein complexes under defined experimental conditions. Although their formation occurs by a self-assembly process, nanodisc purification and the verification of proper reconstitution are still major challenges during the sample preparation. This review gives an overview of the methods used for purifying and analyzing nanodiscs and nanodisc-reconstituted membrane proteins, with an emphasis on the chromatographic and electrophoretic approaches.
Reviews in Analytical Chemistry, 2014, Vol 33, Issue 3, p. 165-172
chromatography; free flow electrophoresis; mass spectrometry; nanodiscs; native PAGE