Bjarnsholt, Thomas4; Alhede, Morten4; Jensen, Peter Østrup5; Nielsen, Anne K.4; Johansen, Helle Krogh2; Homøe, Preben5; Høiby, Niels4; Givskov, Michael Christian4; Kirketerp-Møller, Klaus5
1 Department of Systems Biology, Technical University of Denmark2 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3 Bacterial Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 University of Copenhagen5 Copenhagen University Hospital
Bacterial biofilms are known to be extremely tolerant toward antibiotics and other antimicrobial agents. These biofilms cause the persistence of chronic infections. Since antibiotics rarely resolve these infections, the only effective treatment of chronic infections is surgical removal of the infected implant, tissue, or organ and thereby the biofilm. Acetic acid is known for its antimicrobial effect on bacteria in general, but has never been thoroughly tested for its efficacy against bacterial biofilms. In this article, we describe complete eradication of both Gram-positive and Gram-negative biofilms using acetic acid both as a liquid and as a dry salt. In addition, we present our clinical experience of acetic acid treatment of chronic wounds. In conclusion, we here present the first comprehensive in vitro and in vivo testing of acetic acid against bacterial biofilms.
Advances in Wound Care, 2014, Vol 4, Issue 7, p. 363-372