1 PhD, Department of Nutrition, Exercise and Sports, Faculty of Science, Københavns Universitet2 Integrated Physiology, Department of Nutrition, Exercise and Sports, Faculty of Science, Københavns Universitet3 Molecular Integrative Physiology, Department of Biology, Faculty of Science, Københavns Universitet4 Department of Exercise and Sport Sciences, Faculty of Science, Københavns Universitet5 Integrated Physiology, Department of Nutrition, Exercise and Sports, Faculty of Science, Københavns Universitet6 Molecular Integrative Physiology, Department of Biology, Faculty of Science, Københavns Universitet7 Department of Exercise and Sport Sciences, Faculty of Science, Københavns Universitet
Aim: The polyphenol resveratrol has in animal studies been shown to influence several pathways of importance for angiogenesis in skeletal muscle. The aim was to examine the angiogenic effect of resveratrol supplementation with parallel exercise training in aged men. Methods: Forty-three healthy physically inactive aged men (65±1 years) were divided into A) a training group that conducted 8 weeks of intense exercise training where half of the subjects received a daily intake of either 250 mg trans resveratrol (n=14) and the other half received placebo (n=13); and B) a non-training group that received either 250 mg trans resveratrol (n=9) or placebo (n=7). Results: The group that trained with placebo showed a ~20% increase in capillary to fiber (C:F) ratio, an increase in the muscle protein expression of vascular endothelial growth factor (VEGF), VEGF receptor-2, and tissue inhibitor of matrix metalloproteinase (TIMP-1) but unaltered thrombospodin-1 (TSP-1) levels. Muscle interstitial VEGF and TSP-1 protein levels were unchanged after the training period. The group training with resveratrol supplementation did not show an increase in C:F ratio or an increase in muscle VEGF protein. Muscle TIMP-1 protein levels were lower in the training and resveratrol group than in the training and placebo group. Both training groups showed an increase in Forkhead box O1 protein. In the non-training groups, TIMP-1 protein was lower in the resveratrol group than the placebo group after 8 weeks. Conclusion: These data show that exercise training has a strong angiogenic effect whereas resveratrol supplementation may limit basal and training-induced angiogenesis.
American Journal of Physiology: Heart and Circulatory Physiology, 2014, Vol 307, Issue 8