1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Polymer Microsystems for Medical Diagnostics, Department of Micro- and Nanotechnology, Technical University of Denmark3 Nano Bio Integrated Systems, Department of Micro- and Nanotechnology, Technical University of Denmark4 Copenhagen Center for Health Technology, Center, Technical University of Denmark
This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.
Biosensors, 2014, Vol 4, Issue 3, p. 257-272
Electrical impedance spectroscopy; HeLa cells; Paclitaxel; Cell properties