Michelet, Lorraine4; Delannoy, Sabine4; Devillers, Elodie4; Umhang, Gerald4; Aspan, Anna5; Juremalm, Mikael5; Chirico, Jan5; van der Wal, Fimme Jan6; Pihl, Thomas Peter Boye7; Schou, Kirstine Klitgaard1; Bødker, Rene1; Fach, Patrick4; Moutailler, Sara4
1 National Veterinary Institute, Technical University of Denmark2 Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark3 Section for Epidemiology, National Veterinary Institute, Technical University of Denmark4 Agence nationale de la sécurité sanitaire, alimentation, environnement et travail5 National Veterinary Institute6 Central Veterinary Institute7 Synthetic Biology Tools for Yeast, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens, a large scale epidemiological study was conducted on 7050 Ixodes ricinus nymphs collected from France, Denmark, and the Netherlands using a powerful new high-throughput approach. This advanced methodology permitted the simultaneous detection of 25 bacterial, and 12 parasitic species (including; Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Candidatus Neoehrlichia, Coxiella, Francisella, Babesia, and Theileria genus) across 94 samples. We successfully determined the prevalence of expected (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Rickettsia helvetica, Candidatus Neoehrlichia mikurensis, Babesia divergens, Babesia venatorum), unexpected (Borrelia miyamotoi) and rare (Bartonella henselae) pathogens in the three European countries. Moreover we detected Borrelia spielmanii, Borrelia miyamotoi, Babesia divergens, and Babesia venatorum for the first time in Danish ticks. This surveillance method represents a major improvement in epidemiological studies, able to facilitate comprehensive testing of tick-borne pathogens, and which can also be customized to monitor emerging diseases.
Frontiers in Cellular and Infection Microbiology, 2014, Vol 4, Issue 103