Gold, Marielle C.8; McLaren, James E.9; Reistetter, Joseph A.8; Smyk-Pearson, Sue8; Ladell, Kristin9; Swarbrick, Gwendolyn M.8; Yu, Yik Y. L.5; Hansen, Ted H.5; Lund, Ole6; Nielsen, Morten6; Gerritsen, Bram7; Kesmir, Can7; Miles, John J.9; Lewinsohn, Deborah A.8; Price, David A.9; Lewinsohn, David M.8
1 Department of Systems Biology, Technical University of Denmark2 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark3 Oregon Health and Science University4 Cardiff University5 University of Washington6 Department of Bio and Health Informatics, Technical University of Denmark7 Utrecht University8 Oregon Health and Science University9 Cardiff University
Mucosal-associated invariant T (MAIT) cells express a semi-invariant T cell receptor (TCR) that detects microbial metabolites presented by the nonpolymorphic major histocompatibility complex (MHC)-like molecule MR1. The highly conserved nature of MR1 in conjunction with biased MAIT TCRα chain usage is widely thought to indicate limited ligand presentation and discrimination within a pattern-like recognition system. Here, we evaluated the TCR repertoire of MAIT cells responsive to three classes of microbes. Substantial diversity and heterogeneity were apparent across the functional MAIT cell repertoire as a whole, especially for TCRβ chain sequences. Moreover, different pathogen-specific responses were characterized by distinct TCR usage, both between and within individuals, suggesting that MAIT cell adaptation was a direct consequence of exposure to various exogenous MR1-restricted epitopes. In line with this interpretation, MAIT cell clones with distinct TCRs responded differentially to a riboflavin metabolite. These results suggest that MAIT cells can discriminate between pathogen-derived ligands in a clonotype-dependent manner, providing a basis for adaptive memory via recruitment of specific repertoires shaped by microbial exposure.
Journal of Experimental Medicine, 2014, Vol 211, Issue 8, p. 1601-1610