Petersen, Lene Maj1; Frisvad, Jens Christian3; Larsen, Thomas Ostenfeld4
1 Department of Systems Biology, Technical University of Denmark2 Natural Product Chemistry, Department of Systems Biology, Technical University of Denmark3 Fungal Chemodiversity, Department of Biotechnology and Biomedicine, Technical University of Denmark4 Natural Product Discovery, Department of Biotechnology and Biomedicine, Technical University of Denmark
The black aspergilli constitute an important group of species, both for their use in the biotechnological industry, and since they account for some of the most widespread food and feed contaminants. Most recently we have characterized the full biosynthetic pathway of the antibiotic yanuthone D from the industrially workhorse Aspergillus niger.1 On top of that we have presented a method for sclerotium induction, whereby we have reported sclerotia formation for the first time in seven black aspergilli, including A. niger.2 Thorough investigation by UHPLC-DAD-HRMS of fungal extracts, with and without sclerotia formation, have revealed a highly regulated metabolic profile for a number of species. We present this as an approach for the discovery of novel secondary metabolites from otherwise silent pathways.
Planta Medica, 2014, Vol 80, Issue 10
biotechnological industry; sclerotia formation; unraveling cryptic chemistry; Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes  Aspergillus niger species black aspergilli common; fungal extract; secondary metabolite; yanuthone D antibacterial-drug, antiinfective-drug synthesis; 00520, General biology - Symposia, transactions and proceedings; 12512, Pathology - Therapy; 22002, Pharmacology - General; 38502, Chemotherapy - General, methods and metabolism; 38504, Chemotherapy - Antibacterial agents; 39008, Food microbiology - General and miscellaneous; sclerotium induction method laboratory techniques; UHPLC-DAD-HRMS laboratory techniques, spectrum analysis techniques, chromatographic techniques; Bioprocess Engineering; Pharmacology
Main Research Area:
American Society of Pharmacognosy 2014 Annual Meeting