Shi, Leming4; Haibe-Kains, Benjamin5; Birkbak, Nicolai Juul3; Bedard, Philippe L.5; Hatzis, Christos6; Stern, David F.6; Clarke, Robert9; Quackenbush, John10; Beck, Andrew H.10; Aerts, Hugo J. W. L.10
1 Department of Systems Biology, Technical University of Denmark2 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark3 Cancer Systems Biology, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark4 Zhanjiang Center for Translational Medicine5 University of Toronto6 Yale University7 Georgetown University8 Harvard University9 Georgetown University10 Harvard University
Large-scale pharmacogenomic high-throughput screening (HTS) studies hold great potential for generating robust genomic predictors of drug response. Two recent large-scale HTS studies have reported results of such screens, revealing several known and novel drug sensitivities and biomarkers. Subsequent evaluation, however, found only moderate interlaboratory concordance in the drug response phenotypes, possibly due to differences in the experimental protocols used in the two studies. This highlights the need for community-wide implementation of standardized assays for measuring drug response phenotypes so that the full potential of HTS is realized. We suggest that the path forward is to establish best practices and standardization of the critical steps in these assays through a collective effort to ensure that the data produced from large-scale screens would not only be of high intrastudy consistency, so that they could be replicated and compared successfully across multiple laboratories.
Cancer Research, 2014, Vol 74, Issue 15, p. 4016-4023
drug response phenotype; drug sensitivity; genome; Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae  human common; biomarker; 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines; 12512, Pathology - Therapy; 22002, Pharmacology - General; 22005, Pharmacology - Clinical pharmacology; 24004, Neoplasms - Pathology, clinical aspects and systemic effects; cancer drug screening laboratory techniques; large-scale pharmacogenomic high-throughput screening laboratory techniques; Methods and Techniques; Pharmacology; Tumor Biology; ONCOLOGY; MICROARRAY DATA; CYTOTOXICITY; INHIBITION; DISCOVERY; SENSITIVITY; STANDARDS; MELANOMA; STRATEGY; IMPACT; TARGET; Reviews