Petersen, Lene Maj1; Bladt, Tanja Thorskov1; Duerr, Claudia8; Seiffert, Martina8; Frisvad, Jens Christian6; Gotfredsen, Charlotte Held3; Larsen, Thomas Ostenfeld7
1 Department of Systems Biology, Technical University of Denmark2 Natural Product Chemistry, Department of Systems Biology, Technical University of Denmark3 Department of Chemistry, Technical University of Denmark4 Organic Chemistry, Department of Chemistry, Technical University of Denmark5 German Cancer Research Center (DKFZ)6 Fungal Chemodiversity, Department of Biotechnology and Biomedicine, Technical University of Denmark7 Natural Product Discovery, Department of Biotechnology and Biomedicine, Technical University of Denmark8 German Cancer Research Center (DKFZ)
Two new cytochalasins, sclerotionigrin A (1) and B (2) were isolated together with the known proxiphomin (3) from the filamentous fungus Aspergillus sclerotioniger. The structures and relative stereochemistry of 1 and 2 were determined based on comparison with 3, and from extensive 1D and 2D NMR spectroscopic analysis, supported by high resolution mass spectrometry (HRMS). Compounds 2 and 3 displayed cytotoxic activity towards chronic lymphocytic leukemia cells in vitro, with 3 being the most active.