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1 Department of Systems Biology, Technical University of Denmark 2 Copenhagen University Hospital 3 University of Copenhagen 4 Danish Technological Institute 5 Dansk Fundamental Metrologi A/S 6 Novozymes A/S 7 National Veterinary Institute, Technical University of Denmark 8 Danish Technological Institute
Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro. © 2014 Elsevier Ltd. All rights reserved.
Materials Science and Engineering A: Structural Materials: Properties, Microstructures and Processing, 2014, Vol 43, p. 117-125
Nanocoatings; Osseointegration; Osteoblasts; Rhamnogalacturonan-I; Surface properties; Titanium; Carbohydrate Sequence; Cell Line, Tumor; Cell Proliferation; Coated Materials, Biocompatible; Humans; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Molecular Sequence Data; Nanostructures; Pectins; Photoelectron Spectroscopy; Journal Article
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