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Aspergillus pragensis sp nov discovered during molecular reidentification of clinical isolates belonging to Aspergillus section Candidi

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Authors:
  • Lyskova, Pavlina ;
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    National Institute of Public Health
  • Hubka, Vit ;
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    Academy of Sciences of the Czech Republic
  • Kolarik, Miroslav ;
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    Academy of Sciences of the Czech Republic
  • Skorepova, Magdalena ;
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    General University Hospital
  • Peterson, Stephen W. ;
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    United States Department of Agriculture
  • Frisvad, Jens Christian
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    Orcid logo0000-0002-0573-4340
    Natural Product Chemistry, Department of Systems Biology, Technical University of Denmark
DOI:
10.1093/mmy/myu022
Abstract:
The identity of nine clinical isolates recovered from Czech patients and presumptively identified as Aspergillus sp. section Candidi based on colony morphology was revised using sequences of beta-tubulin, calmodulin gene sequence, and internal transcribed spacer rDNA. Six isolates were from suspected and proven onychomycosis, one from otitis externa, and two associated with probable invasive aspergillosis. The results showed that one Aspergillus candidus isolate was the cause of otitis externa, and both isolates obtained from sputa of patients with probable invasive aspergillosis were reidentified as A. carneus (sect. Terrei) and A. flavus (sect. Flavi). Three isolates from nail scrapings were identified as A. tritici, a verified agent of nondermatophyte onychomycosis. One isolate from toenail was determined to be A. candidus and the two isolates belonged to a hitherto undescribed species, Aspergillus pragensis sp. nov. This species is well supported by phylogenetic analysis based on beta-tubulin and calmodulin gene and is distinguishable from other members of sect. Candidi by red-brown reverse on malt extract agar, slow growth on Czapek-Dox agar and inability to grow at 37 degrees C. A secondary metabolite analysis was also provided with comparison of metabolite spectrum to other species. Section Candidi now encompasses five species for which a dichotomous key based on colony characteristics is provided. All clinical isolates were tested for susceptibilities to selected antifungal agents using the Etest and disc diffusion method. Overall sect. Candidi members are highly susceptible to common antifungals.
Type:
Journal article
Language:
English
Published in:
Medical Mycology, 2014, Vol 52, Issue 6, p. 565-576
Keywords:
Czech Republic Europe Palearctic region; invasive aspergillosis Aspergillosis (MeSH) fungal disease epidemiology; onychomycosis Onychomycosis (MeSH) integumentary system disease, fungal disease; otitis Otitis (MeSH) ear disease; Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500*] Aspergillus flavus species Aspergillus carneus species Aspergillus pragensis species new species pathogen description Aspergillus Candidi species pathogen; Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common host; Aspergillus carneus Bt2a gene [Fungi Imperfecti or Deuteromycetes] expression; Aspergillus pragensis beta-tubulin gene [Fungi Imperfecti or Deuteromycetes] expression; Aspergillus pragensis calmodulin gene [Fungi Imperfecti or Deuteromycetes] expression; internal transcribed spacer rDNA; 00504, General biology - Taxonomy, nomenclature and terminology; 03502, Genetics - General; 03504, Genetics - Plant; 03508, Genetics - Human; 11102, Anatomy and Histology - Gross anatomy; 12502, Pathology - General; 18506, Integumentary system - Pathology; 20006, Sense organs - Pathology; 36008, Medical and clinical microbiology - Mycology; 50506, Botany: general and systematic - Fungi; 51000, Morphology, anatomy and embryology of plants; 62520, Chordata: general and systematic - Mammalia; Biochemistry and Molecular Biophysics; Human Medicine, Medical Sciences; phylogenetic analysis mathematical and computer techniques; Infection; Molecular Genetics; Otolaryngology; Systematics and Taxonomy; MYCOLOGY; VETERINARY; TAXONOMIC REVISION; PHYSIOLOGICAL DATA; DNA-SEQUENCES; COLOR MUTANTS; ONYCHOMYCOSIS; TAICHUNGENSIS; METABOLITES; FUMIGATUS; PENICILLIUM; INFECTIONS; Aspergillus candidus; Aspergillus tritici; antifungal susceptibility testing; nondermatophyte onychomycosis; otomycosis; polyphasic approach
Main Research Area:
Science/technology
Publication Status:
Published
Review type:
Peer Review
Submission year:
2014
Scientific Level:
Scientific
ID:
268512900

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