1 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet 2 Department of Chemistry, Faculty of Science, Københavns Universitet 3 Department of Chemistry, Faculty of Science, Københavns Universitet 4 Administration, Department of Chemistry, Faculty of Science, Københavns Universitet
effect of deposition temperature
We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (Tm) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above Tmby neutron reflection and investigated the effect of subsequent cooling to below the Tm. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid Tm. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported. © 2014 American Chemical Society.
Langmuir, 2014, Vol 30, Issue 25, p. 7259-7263
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