In this work we present a theoretical, computational, and experimental evaluation of the performance of an impedimetric biosensor based on interdigitated conductive polymer (PEDOT:TsO) microelectrodes in a microfluidic system. The influence of the geometry of the electrodes and microchannels on the electrochemical performance of the biosensor was exploited to improve the detection system. The developed model allowed us to predict the performance of the electrochemical system, and thus to optimize the geometry for electrochemical impedance spectroscopy (EIS). Finally, the optimized electrode design was validated by the detection of a clinically relevant target (ampicillin) at picomolar concentrations.
Electroanalysis, 2014, Vol 26, Issue 6, p. 1400-1408