; ; ;
1 Department of Chemical and Biochemical Engineering, Technical University of Denmark 2 Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark 3 Johann Heinrich von Thünen-Institute 4 University of Hamburg
The monosaccharide composition of four different samples of brown seaweeds Laminaria digitata and Saccharina latissima were compared by different high performance anion exchange chromatography (HPAEC) methods after different acid hydrolysis treatments or a cellulase treatment. A two-step treatment of 72% (w/w) H2SO4 + 4% (w/w) H2SO4 performed best, but cellulase treatment released more glucose than acid treatments. HPAEC with pulsed amperometric detection (PAD) allowed quantification of all present neutral sugars and the sugar alcohol mannitol. Furthermore, the use of guluronic, glucuronic, and galacturonic acid as standards enabled quantification of the uronic acids. A complete map of amino acids, fatty compounds, minerals, and ash was also achieved. L. digitata and S. latissima harvested in Denmark April (Baltic Sea, 2012) were dominated by alginic acid and ash (each ∼30% by weight (w/w) of the dry matter) and 10% (w/w) protein. In contrast, the dominant compound of L. digitata harvested in August (North Sea, 2012) was glucose constituting 51% w/w of the dry matter, and with 16% w/w alginic acid. Washing prior to analysis mainly removed salts. © the Partner Organisations 2014.
R S C Advances, 2014, Vol 4, Issue 49, p. 25736-25746
Chemical Engineering (all); Chemistry (all); Amino acids; Glucose; Seaweed; Sodium alginate; Carbohydrate compositions; Galacturonic acids; High-performance anion-exchange chromatography; Laminaria digitata; Monosaccharide composition; Pulsed amperometric detection; Quantitative determinations; Saccharina latissima; Fatty acids
Main Research Area: