Verspreet, Joran5; Hansen, Anders Holmgaard1; Dornez, Emmie5; Courtin, Christophe M.5; Harrison, Scott James4
1 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2 iLoop, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3 KU Leuven4 CFB - Core Flow, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5 KU Leuven
In this paper, a new liquid chromatography-mass spectrometry (LC-MS) method for the analysis of complex fructan mixtures is presented. In this method, columns with a trifunctional C18 alkyl stationary phase (T3) were used and their performance compared with that of a porous graphitized carbon (PGC) column. The separation of fructan isomers with the T3 phase improved clearly in comparison with the PGC phase, and retention times were lower and more stable. When the T3-based method was applied on a wheat grain extract, multiple fructan isomers could be discerned, even for fructans with a degree of polymerization of 10. This indicates that wheat grain fructans do not, or not only, have a simple linear structure. The presented method paves the way for elucidation of fructan structures in complex mixtures that contain many structural isomers.
Analytical and Bioanalytical Chemistry, 2014, Vol 406, Issue 19, p. 4785-4788