1 Department of Systems Biology, Technical University of Denmark2 Infection Microbiology, Department of Systems Biology, Technical University of Denmark3 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 Bacterial Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5 Infection Microbiology, Department of Biotechnology and Biomedicine, Technical University of Denmark
Small regulatory RNAs (sRNAs) in bacteria are known to modulate gene expression and control a variety of processes including metabolic reactions, stress responses, and pathogenesis in response to environmental signals. A method to identify bacterial sRNAs on a genome-wide scale based on RNA sequencing (RNA-seq) is described that involves the preparation and analysis of three different sequencing libraries. As a signifi cant number of unique sRNAs are identifi ed in each library, the libraries can be used either alone or in combination to increase the number of sRNAs identifi ed. The approach may be applied to identify sRNAs in any bacterium under different growth and stress conditions.