Liengaard, Lars2; Figueiredo, Viviane3; Markfoged, Rikke4; Revsbech, Niels Peter5; Nielsen, Lars Peter6; Prast, Alex E.3; Kühl, Michael7
1 Marine Biology, Department of Biology, Faculty of Science, Københavns Universitet2 Biologisk Institut, Københavns Universitet3 University Federal of Rio de Janeiro4 Mikrobiologi, Biologisk Institut5 Interdisciplinary Nanoscience Center - INANO-Bioscience, Ny Munkegade6 Institut for Bioscience - Mikrobiologi7 Marine Biology, Department of Biology, Faculty of Science, Københavns Universitet
Tropical wetland soils emit large amounts of nitrous oxide (N2O), especially following wetting of drained soil. We investigated seasonally drained wetland soils from the Pantanal and the Amazon, both with a natural high nitrate content and low pH. Here we report the effect of wetting on the production, emission and consumption of N2O on these soils. Intact soil cores were wetted to simulate natural water logging events, and microsensor measurements were used to i) characterize the vertical microscale distribution of O2 and N2O, ii) monitor the accumulation of N2O in the anoxic soil volume, and iii) quantify the N2O efflux out of the soil. Flux chamber measurements of N2O emission confirmed the effluxes calculated from microsensor measurements. The N2O concentration dynamics in the soil cores were characterized by three distinct phases: 1) an initial slow N2O production, 2) a higher N2O production ending abruptly when the supply of NO3 and NO2 (NOx) was exhausted, and 3) a final phase where the accumulated N2O was reduced to N2. This evolution of the N2O pool in an intact soil core could be accurately simulated by a simple diffusionreaction model with the presence of O2 and NOx as determining factors. Approximately one third of the initial NO3 present in the soil was lost as N2O or N2. As the soil was depleted for NO3 by the end of the experiment we suggest that dissimilatory nitrate reduction to ammonia (DNRA) was responsible for reducing the remaining NO3 .
Soil Biology and Biochemistry, 2014, Vol 75, p. 26-36