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Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality

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Authors:
  • Richard, Nadege ;
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    Universidade do Algarve
  • Fernandez, Ignacio ;
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    Universidade do Algarve
  • Wulff, Tune ;
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    Orcid logo0000-0002-8822-1048
    National Food Institute, Technical University of Denmark
  • Hamre, Kristin ;
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    National Institute for Nutrition and Seafood Research
  • Cancela, Leonor ;
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    Universidade do Algarve
  • Conceicao, Luis E. C. ;
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    Universidade do Algarve
  • Gavaia, Paulo J.
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    Universidade do Algarve
DOI:
10.1007/s10126-014-9571-2
Abstract:
Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr). Since the mechanisms mediating VK effects over skeletal development are poorly known, we investigated the effects of VK-supplementation on skeletal development in Senegalese sole larvae, aiming to identify molecular pathways involved. Larvae were fed live preys enriched with graded levels of phylloquinone (PK) (0, 50, and 250 mg kg(-1)) and survival rate, growth, VK contents, calcium content and incidence of skeletal deformities were determined, revealing an improvement of larval performance and decreasing the incidence of deformities in VK-supplemented groups. Comparative proteome analysis revealed a number of differentially expressed proteins between Control and Diet 250 associated with key biological processes including skin, muscle, and bone development. Expression analysis showed that genes encoding proteins related to the VK cycle (ggcx, vkor), VK nuclear receptor (pxr), and VK-dependent proteins (VKDPs; oc1 and grp), were differentially expressed. This study highlights the potential benefits of increasing dietary VK levels in larval diets, and brings new insights on the mechanisms mediating the positive effects observed on larval performance and skeletal development.
Type:
Journal article
Language:
English
Published in:
Marine Biotechnology, 2014, Vol 16, Issue 5, p. 522-537
Keywords:
BIOTECHNOLOGY; MARINE; SALMO-SALAR L.; NICOTINAMIDE BISULFITE VITAMIN-K-3; MUMMICHOG FUNDULUS-HETEROCLITUS; XENOBIOTIC RECEPTOR SXR; HEAVY-CHAIN GENES; GLA-RICH PROTEIN; REAL-TIME PCR; ATLANTIC SALMON; MOLECULAR CHARACTERIZATION; VASCULAR CALCIFICATION; Vitamin K; Skeletal deformities; Proteomics; Gene expression; Solea senegalensis; Flatfish larvae; HASH(0x3cc4408)
Main Research Area:
Science/technology
Publication Status:
Published
Review type:
Peer Review
Submission year:
2014
Scientific Level:
Scientific
ID:
267101637

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