Bjørnbak, Camilla3; Brøchner, Christian B4; Larsen, Lars A4; Johansen, Julia S1; Møllgård, Kjeld4
1 Internal Medicine, Herlev, Herlev and Gentofte Hospital, The Capital Region of Denmark2 Oncology, Herlev and Gentofte Hospital, The Capital Region of Denmark3 Institut for Cellulær og Molekylær Medicin, Afdeling VI4 unknown
YKL-40, a glycoprotein involved in cell differentiation, has been associated with neurodevelopmental disorders, angiogenesis, neuroinflammation and glioblastomas. We evaluated YKL-40 protein distribution in the early human forebrain using double-labeling immunofluorescence and immunohistochemistry. Immunoreactivity was detected in neuroepithelial cells, radial glial end feet, leptomeningeal cells and choroid plexus epithelial cells. The subpial marginal zone was YKL-40-positive, particularly in the hippocampus, from an early beginning stage in its development. Blood vessels in the intermediate and subventricular zones showed specific YKL-40 reactivity confined to pericytes. Furthermore, a population of YKL-40-positive, small, rounded cells was identified in the ventricular and subventricular zones. Real-time quantitative RT-PCR analysis showed strong YKL-40 mRNA expression in the leptomeninges and the choroid plexuses, and weaker expression in the telencephalic wall. Immunohistochemistry revealed a differential distribution of YKL-40 across the zones of the developing telencephalic wall. We show that YKL-40 is associated with sites of the brain barrier systems and propose that it is involved in controlling local angiogenesis and access of peripheral cells to the forebrain via secretion from leptomeningeal cells, choroid plexus epithelium and pericytes. Furthermore, we suggest that the small, rounded, YKL-40-positive cells represent a subpopulation of astroglial progenitors, and that YKL-40 could be involved in the differentiation of a particular astrocytic lineage.
Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 2014, Vol 62, Issue 5, p. 369-88
Journal Article; Research Support, Non-U.S. Gov't; Adipokines; Astrocytes; Base Sequence; DNA Primers; Humans; Immunohistochemistry; Lectins; Microscopy, Confocal; Neural Stem Cells; Prosencephalon; RNA, Messenger; Real-Time Polymerase Chain Reaction