Equbal, Asif2; Paul, Subhradip3; Mithu, Venus Singh4; Vinther, Joachim Møllesøe7; Nielsen, Niels Chr8; Madhu, P K6
1 Natural Products and Peptides, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet2 Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience, Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark.3 Department of Chemistry, Center for Excellence in Basic Sciences, University of Mumbai and Department of Atomic Energy, Health Centre, University of Mumbai, Vidhyanagari Campus, Mumbai 400098, India.4 Department of Chemistry, Guru Nanak Dev University, Amritsar 143005, India.5 Department of Drug Design and Pharmacology, Faculty of Pharmaceutical Sciences, Københavns Universitet6 Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India; TIFR Centre for Interdisciplinary Sciences, 21 Brundavan Colony, Narsingi, Hyderabad 500075, India. Electronic address: email@example.com Natural Products and Peptides, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet8 Department of Drug Design and Pharmacology, Faculty of Pharmaceutical Sciences, Københavns Universitet
Towards improving solid-state NMR two-pulse phase-modulation heteronuclear dipolar decoupling sequence by refocusing
We present here a simple refocused modification, r TPPM, of the Two-Pulse Phase-Modulation (TPPM) heteronuclear decoupling method, which improves decoupling and makes the sequence much more robust with respect to essential experimental parameters. The modified sequence is compared with the established TPPM sequence and a variety of other decoupling sequences at low to moderate magic-angle spinning frequencies. Simulations are shown to compare TPPM and r TPPM with respect to various experimental parameters. The observations from simulations are corroborated with experimental findings at two spinning frequencies on U-(13)C-glycine and U-(13)C-L-histidine.HCl.H2O.
Journal of Magnetic Resonance, 2014, Vol 244, p. 68-73