The cell-free fetal DNA fraction in maternal blood decreases after physical activity

Jacob Mørup Schlütter; Lotte Hatt; Cathrine Bach; Ida Kirkegaard; Steen Kølvraa; Niels Uldbjerg

doi:10.1002/pd.4306

The cell-free fetal DNA fraction in maternal blood decreases after physical activity

Authors:

Schlütter, Jacob Mørup ;
Close
0000-0002-2045-1007
Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University
Hatt, Lotte ;
Close
Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University
Bach, Cathrine ;
Close
Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University
Kirkegaard, Ida ;
Close
Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University
Kølvraa, Steen ;
Close
Department of Human Genetics, Faculty of Health Sciences, Aarhus University, Aarhus University
Uldbjerg, Niels
Close
0000-0002-6449-6426
Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University

DOI:

10.1002/pd.4306

Abstract:

OBJECTIVE: If noninvasive prenatal testing using next generation sequencing is to be effective for pregnant women, a cell-free fetal DNA (cffDNA) fraction above 4% is essential unless the depth of sequencing is increased. This study's objective is to determine whether physical activity has an effect on the proportion of cell-free DNA (cfDNA) arising from the fetus (fetal fraction). METHODS: Nine pregnant women carrying male fetuses at gestational age 12(+0) weeks to 14(+6) weeks were included. Plasma from nine pregnant women was drawn prior to, immediately after, and 30 min after 30 min of cycling with a pulse-rate of 150 beats per minute. The concentrations of cffDNA (DYS14) and cfDNA (RASSF1A) were assessed using quantitative real-time polymerase chain reaction. RESULTS: The fetal fraction decreased significantly in all participants after physical activity (p < 0.01), a decrease varying from 1-17 percentage points. This was due to a significant increase in the concentration of cfDNA (p < 0.01), whereas the concentration of cffDNA remained the same. This alteration of the fetal fraction was not present 30 min after physical activity. CONCLUSION: When planning the timing of noninvasive prenatal diagnosis based on the fetal fraction, physical activity prior to sampling should be avoided.

Type:

Journal article

Language:

English

Published in:

Prenatal Diagnosis, 2014, Vol 34, Issue 4, p. 341-4

Main Research Area:

Medical science

Publication Status:

Published

Review type:

Peer Review

Submission year:

2014

Scientific Level:

Scientific

ID:

261064596

The cell-free fetal DNA fraction in maternal blood decreases after physical activity

Full text access

On-site access

At institutions

Metrics

Example	Finds records
water{}	containing the word "water".
water supplies"{}"	containing the phrase "water supplies".
author:"Doe, John"author:"{}"	containing the prase "Doe, John" in the author field.
title:IEEEtitle:{}	containing the word "IEEE" in the title field.
Need more help?	Advanced search tutorial