van der Poel, W. H. M.3; Cay, B.4; Zientara, S.5; Steinbach, F.6; Valarcher, J. F.7; Bøtner, Anette1; Mars, M. H.8; Hakze-van der Honing, R.3; Schirrmeier, H.9; Beer, M.9
1 National Veterinary Institute, Technical University of Denmark2 Section for Virology, National Veterinary Institute, Technical University of Denmark3 Central Veterinary Institute4 Centrum voor Onderzoek in Diergeneeskunde en Agrochemie5 French Agency for Food, Environmental and Occupational Health & Safety6 Animal Health and Veterinary Laboratories Agency7 National Veterinary Institute8 Animal Health Service9 Friedrich Loeffler Institute
Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.