1 Department of Environmental Engineering, Technical University of Denmark2 Urban Water Engineering, Department of Environmental Engineering, Technical University of Denmark3 University of Copenhagen
Microbial communities derived from soils subject to different agronomic treatments were challenged with three broad host range plasmids, RP4, pIPO2tet and pRO101, via solid surface filter matings to assess their permissiveness. Approximately 1 in 10 000 soil bacterial cells could receive and maintain the plasmids. The community permissiveness increased up to 100% in communities derived from manured soil. While the plasmid transfer frequency was significantly influenced by both the type of plasmid and the agronomic treatment, the diversity of the transconjugal pools was purely plasmid dependent and was dominated by β- and γ-Proteobacteria.
Environmental Microbiology Reports, 2014, Vol 6, Issue 2, p. 125-130
SOIL microbiology; GenBank sequence data; manure exposure; soil bacterial community potential; Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria  beta-Proteobacteria higher_taxa gamma-Proteobacteria higher_taxa; pIPO2tet; plasmid; pRO101; RP4; 03502, Genetics - General; 07502, Ecology: environmental biology - General and methods; 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines; 30000, Bacteriology, general and systematic; 31000, Physiology and biochemistry of bacteria; 31500, Genetics of bacteria and viruses; Biochemistry and Molecular Biophysics; agronomic treatment applied and field techniques; Biodiversity; Molecular Genetics; ENVIRONMENTAL; MICROBIOLOGY; BROAD-HOST-RANGE; HORIZONTAL GENE-TRANSFER; RESISTANT BACTERIA; WHEAT RHIZOSPHERE; PERMISSIVENESS; FERTILIZATION; PSEUDOMONAS; RESIDUES; ELEMENTS