Jensen, Pernille Linnert3; Grøndahl, Marie Louise8; Beck, Hans Christian5; Petersen, Jørgen6; Stroebech, Lotte7; Christensen, Søren Tvorup9; Andersen, Claus Yding10
1 Cell Biology and Physiology, Department of Biology, Faculty of Science, Københavns Universitet2 Department of Clinical Medicine, Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet3 University Hospital of Copenhagen4 Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet5 Odense University Hospital6 Institut for Biokemi og Molekylær Biologi7 Origio A/S8 Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet9 Cell Biology and Physiology, Department of Biology, Faculty of Science, Københavns Universitet10 Department of Clinical Medicine, Department of Clinical Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
Abstract The understanding of the early mammalian development is a prerequisite for the advancement of in vitro fertilization and improvement of derivation and culturing of embryonic stem cells. While, whole genome transcriptomic analysis on bovine blastocysts has identified genes active in early development, little information is available about the protein complement of early embryos. Modern, sensitive proteomic technology (nano HPLC tandem mass spectrometry) allowed us to describe the proteome of the scarce blastocoel fluid and cell material of expanded bovine blastocysts isolated by micromanipulation. From two independent replicates, 23 proteins were identified in the blastocoel fluid while 803 proteins were identified in the remaining cell material. The proteins were grouped into categories according to their gene ontology (GO) terms by which proteins involved in cell differentiation, cell proliferation, development, and reproduction could be derived. Proteins classified in these categories could be candidates for further functional studies to understand pluripotency and early mammalian development.
Systems Biology in Reproductive Medicine, 2014, Vol 60, Issue 3, p. 127-135