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1 Department of Micro- and Nanotechnology, Technical University of Denmark 2 Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark 3 Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark 4 Politecnico di Milano 5 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark
A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. These widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10 μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. The characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100 kHz vs. 24 kHz) shifting over time. © 2014 Taylor & Francis Group.
Lecture Notes on Impedance Spectroscopy: Measurement, Modeling and Applications, 2013, p. 75-82
Cell adhesion; Cell culture; Cell proliferation; Spectroscopy; Voltage regulators; Microelectrodes
Main Research Area:
6th International Workshop on Impedance Spectroscopy, 2013